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KMID : 0376319960080010081
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Dental Journal of CNU
1996 Volume.8 No. 1 p.81 ~ p.90
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Inhibition of artificial plaque by mutanase produced from Streptomyces exfoliatus
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Song Do-Won
Yang Kyu-Ho
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Abstract
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The main component of dental plaque is the mutan containing the α-1, 3 bond. The following results were obtained by using a blue mutan to assess the factors affecting the mutan-digesting activity of Streptomyces exfoliatus isolated from soil.
A clear zone was produced by mutanase-producing Streptomyces exfoliatus on the minimal essential agar containing blue mutan. Streptomyces exfoliatus digested more blue mutan in the minimal essential broth at pH 7.0 than at pH 5.5 or 8.5. Streptomyces exfoliatus digested more blue mutan at 37℃ than at 32℃ or 42℃(P<0.05). When the concentration of CaCl_2 was increased in the minimal essential broth, the digestion of blue mutan was increased(P<0.05). The optimal concentration of KCl was 10mM to digest blue mutan, but a similar amount of blue mutan was digested at the range of 0.1mM to 6.4mM of MgCl_2. When the culture supernatant of Streptomyces exfoliatus was mixed with 2X brain heart infusion broth containing 0.5% yeast extract and 10% sucrose, less artificial plaque was formed by Streptococcus mutans on the orthodontic wire(P<0.05).
These results indicated that the secretion of mutanase was identified in culture supernatant of mutan-digesting Streptomyces exfoliatus, suppressing the formation of artificial plaque by Streptococcus mutans.
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